(BLS) BIG LIVER AND SPLEEN DISEASE ANTIBODY Test Kit
The BLS ELISA kit will measure the amount of antibody to BLS (Avian Hepevirus) in the serum of chickens. Microtitre plates have been pre-coated with inactivated BLS antigen. Chicken serum samples are diluted and added to the microtitre wells where any anti-BLS antibodies present will bind and form an antigen-antibody complex. Non-specific antibodies and other serum proteins are then washed away. Anti-chicken IgG labelled with the enzyme alkaline phosphatase is then added to the wells and binds to any chicken anti-BLS antibodies bound to the antigen. After another wash to remove unreacted conjugate, substrate is added in the form of pNPP chromogen. A yellow colour is developed if anti-BLS antibody is present and the intensity is directly related to the amount of anti-BLS present in the sample.