(Mm) MYCOPLASMA MELEAGRIDIS ANTIBODY Test Kit
The Mm ELISA kit will measure the amount of antibody to Mm in the serum of turkeys. Microtitre plates have been pre-coated with inactivated Mm antigen. Turkey serum samples are diluted and added to the microtitre wells where any anti-Mm antibodies present will bind and form an antigen-antibody complex. Non-specific antibodies and other serum proteins are then washed away. Anti-chicken IgG labelled with the enzyme alkaline phosphatase is then added to the wells and binds to any turkey anti-Mm antibodies originally bound to the antigen. After another wash to remove unreacted conjugate, substrate is added in the form of pNPP chromogen. A yellow colour is developed if anti-Mm antibody is present and the intensity is directly related to the amount of anti-Mm present in the sample.