(REV) RETICULOENDOTHELIOSIS VIRUS ANTIBODY Test Kit
Gamintojo informacija
The REV ELISA kit will measure the amount of antibody to REV (Avian Reticuloendotheliosis virus) in the serum of chickens. Microtitre plates have been pre-coated with inactivated REV antigen. Serum samples are diluted and added to the microtitre wells where any anti-REV antibodies present will bind and form an antigen-antibody complex. Non-specific antibodies and other serum proteins are then washed away. Anti-chicken IgG labelled with the enzyme alkaline phosphatase is then added to the wells and binds to any chicken anti-REV antibodies originally bound to the antigen. After another wash to remove unreacted conjugate, substrate is added in the form of pNPP chromogen. A yellow colour is developed if anti-REV antibody is present and the intensity is directly related to the amount of anti-REV present in the sample.